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primary antibodies against human trpm8  (Alomone Labs)


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    Alomone Labs primary antibodies against human trpm8
    (A) Western blot analysis indicating that overexpression sM8α-His fusion protein reduced the expression of p-JNK and increased the expression of active MMP-2 (64 kDa) in LNCaP cells. GAPDH was used as the inner control. (B) sM8α-His fusion protein was significantly overexpressed in LNCaP-sM8α cells. (C) The expression of p-JNK protein was significantly reduced in LNCaP-sM8α cells compared with LNCaP and LNCaP-NC cells. However, there were no significant changes in the expression of (D) p-ERK1/2 and (F) p-p38 or (E) full-length <t>TRPM8</t> protein. (G) MMP-2 was activated, as indicated by upregulation of 64-kDa MMP-2 and downregulation of 72-kDa MMP-2 in LNCaP-sM8α cells. (H) There were no remarkable differences in the expression of MMP-9 in LNCaP, LNCaP-NC and LNCaP-sM8α cells. *P<0.05. NC, negative control; sM8α, short transient receptor potential melastatin 8α; TRPM8, transient receptor potential melastatin 8; p-ERK, phosphorylated-extracellular signal-regulated kinase 1/2; p-JNK, phosphorylated-c-Jun N-terminal kinase; MMP, matrix metalloproteinase; GAPDH, glyceraldehyde phosphate dehydrogenase.
    Primary Antibodies Against Human Trpm8, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 95/100, based on 43 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/primary+antibodies+against+human+trpm8/pmc04533716-206-0-10?v=Alomone+Labs
    Average 95 stars, based on 43 article reviews
    primary antibodies against human trpm8 - by Bioz Stars, 2026-07
    95/100 stars

    Images

    1) Product Images from "Overexpression of short TRPM8 variant α promotes cell migration and invasion, and decreases starvation-induced apoptosis in prostate cancer LNCaP cells"

    Article Title: Overexpression of short TRPM8 variant α promotes cell migration and invasion, and decreases starvation-induced apoptosis in prostate cancer LNCaP cells

    Journal: Oncology Letters

    doi: 10.3892/ol.2015.3373

    (A) Western blot analysis indicating that overexpression sM8α-His fusion protein reduced the expression of p-JNK and increased the expression of active MMP-2 (64 kDa) in LNCaP cells. GAPDH was used as the inner control. (B) sM8α-His fusion protein was significantly overexpressed in LNCaP-sM8α cells. (C) The expression of p-JNK protein was significantly reduced in LNCaP-sM8α cells compared with LNCaP and LNCaP-NC cells. However, there were no significant changes in the expression of (D) p-ERK1/2 and (F) p-p38 or (E) full-length TRPM8 protein. (G) MMP-2 was activated, as indicated by upregulation of 64-kDa MMP-2 and downregulation of 72-kDa MMP-2 in LNCaP-sM8α cells. (H) There were no remarkable differences in the expression of MMP-9 in LNCaP, LNCaP-NC and LNCaP-sM8α cells. *P<0.05. NC, negative control; sM8α, short transient receptor potential melastatin 8α; TRPM8, transient receptor potential melastatin 8; p-ERK, phosphorylated-extracellular signal-regulated kinase 1/2; p-JNK, phosphorylated-c-Jun N-terminal kinase; MMP, matrix metalloproteinase; GAPDH, glyceraldehyde phosphate dehydrogenase.
    Figure Legend Snippet: (A) Western blot analysis indicating that overexpression sM8α-His fusion protein reduced the expression of p-JNK and increased the expression of active MMP-2 (64 kDa) in LNCaP cells. GAPDH was used as the inner control. (B) sM8α-His fusion protein was significantly overexpressed in LNCaP-sM8α cells. (C) The expression of p-JNK protein was significantly reduced in LNCaP-sM8α cells compared with LNCaP and LNCaP-NC cells. However, there were no significant changes in the expression of (D) p-ERK1/2 and (F) p-p38 or (E) full-length TRPM8 protein. (G) MMP-2 was activated, as indicated by upregulation of 64-kDa MMP-2 and downregulation of 72-kDa MMP-2 in LNCaP-sM8α cells. (H) There were no remarkable differences in the expression of MMP-9 in LNCaP, LNCaP-NC and LNCaP-sM8α cells. *P<0.05. NC, negative control; sM8α, short transient receptor potential melastatin 8α; TRPM8, transient receptor potential melastatin 8; p-ERK, phosphorylated-extracellular signal-regulated kinase 1/2; p-JNK, phosphorylated-c-Jun N-terminal kinase; MMP, matrix metalloproteinase; GAPDH, glyceraldehyde phosphate dehydrogenase.

    Techniques Used: Western Blot, Over Expression, Expressing, Negative Control



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    primary antibodies against human trpm8  (Alomone Labs)
    95
    Alomone Labs primary antibodies against human trpm8
    (A) Western blot analysis indicating that overexpression sM8α-His fusion protein reduced the expression of p-JNK and increased the expression of active MMP-2 (64 kDa) in LNCaP cells. GAPDH was used as the inner control. (B) sM8α-His fusion protein was significantly overexpressed in LNCaP-sM8α cells. (C) The expression of p-JNK protein was significantly reduced in LNCaP-sM8α cells compared with LNCaP and LNCaP-NC cells. However, there were no significant changes in the expression of (D) p-ERK1/2 and (F) p-p38 or (E) full-length <t>TRPM8</t> protein. (G) MMP-2 was activated, as indicated by upregulation of 64-kDa MMP-2 and downregulation of 72-kDa MMP-2 in LNCaP-sM8α cells. (H) There were no remarkable differences in the expression of MMP-9 in LNCaP, LNCaP-NC and LNCaP-sM8α cells. *P<0.05. NC, negative control; sM8α, short transient receptor potential melastatin 8α; TRPM8, transient receptor potential melastatin 8; p-ERK, phosphorylated-extracellular signal-regulated kinase 1/2; p-JNK, phosphorylated-c-Jun N-terminal kinase; MMP, matrix metalloproteinase; GAPDH, glyceraldehyde phosphate dehydrogenase.
    Primary Antibodies Against Human Trpm8, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/primary+antibodies+against+human+trpm8/pmc04533716-206-0-10?v=Alomone+Labs
    Average 95 stars, based on 1 article reviews
    primary antibodies against human trpm8 - by Bioz Stars, 2026-07
    95/100 stars
    		  Buy from Supplier
    primary rabbit polyclonal antibodies against human trpm8/trpa1  (Thermo Fisher)
    90
    Thermo Fisher primary rabbit polyclonal antibodies against human trpm8/trpa1
    (A) Western blot analysis indicating that overexpression sM8α-His fusion protein reduced the expression of p-JNK and increased the expression of active MMP-2 (64 kDa) in LNCaP cells. GAPDH was used as the inner control. (B) sM8α-His fusion protein was significantly overexpressed in LNCaP-sM8α cells. (C) The expression of p-JNK protein was significantly reduced in LNCaP-sM8α cells compared with LNCaP and LNCaP-NC cells. However, there were no significant changes in the expression of (D) p-ERK1/2 and (F) p-p38 or (E) full-length <t>TRPM8</t> protein. (G) MMP-2 was activated, as indicated by upregulation of 64-kDa MMP-2 and downregulation of 72-kDa MMP-2 in LNCaP-sM8α cells. (H) There were no remarkable differences in the expression of MMP-9 in LNCaP, LNCaP-NC and LNCaP-sM8α cells. *P<0.05. NC, negative control; sM8α, short transient receptor potential melastatin 8α; TRPM8, transient receptor potential melastatin 8; p-ERK, phosphorylated-extracellular signal-regulated kinase 1/2; p-JNK, phosphorylated-c-Jun N-terminal kinase; MMP, matrix metalloproteinase; GAPDH, glyceraldehyde phosphate dehydrogenase.
    Primary Rabbit Polyclonal Antibodies Against Human Trpm8/Trpa1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/primary+antibodies+against+human+trpm8/pm28043013-68-7-27?v=Thermo+Fisher
    Average 90 stars, based on 1 article reviews
    primary rabbit polyclonal antibodies against human trpm8/trpa1 - by Bioz Stars, 2026-07
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    (A) Western blot analysis indicating that overexpression sM8α-His fusion protein reduced the expression of p-JNK and increased the expression of active MMP-2 (64 kDa) in LNCaP cells. GAPDH was used as the inner control. (B) sM8α-His fusion protein was significantly overexpressed in LNCaP-sM8α cells. (C) The expression of p-JNK protein was significantly reduced in LNCaP-sM8α cells compared with LNCaP and LNCaP-NC cells. However, there were no significant changes in the expression of (D) p-ERK1/2 and (F) p-p38 or (E) full-length TRPM8 protein. (G) MMP-2 was activated, as indicated by upregulation of 64-kDa MMP-2 and downregulation of 72-kDa MMP-2 in LNCaP-sM8α cells. (H) There were no remarkable differences in the expression of MMP-9 in LNCaP, LNCaP-NC and LNCaP-sM8α cells. *P<0.05. NC, negative control; sM8α, short transient receptor potential melastatin 8α; TRPM8, transient receptor potential melastatin 8; p-ERK, phosphorylated-extracellular signal-regulated kinase 1/2; p-JNK, phosphorylated-c-Jun N-terminal kinase; MMP, matrix metalloproteinase; GAPDH, glyceraldehyde phosphate dehydrogenase.

    Journal: Oncology Letters

    Article Title: Overexpression of short TRPM8 variant α promotes cell migration and invasion, and decreases starvation-induced apoptosis in prostate cancer LNCaP cells

    doi: 10.3892/ol.2015.3373

    Figure Lengend Snippet: (A) Western blot analysis indicating that overexpression sM8α-His fusion protein reduced the expression of p-JNK and increased the expression of active MMP-2 (64 kDa) in LNCaP cells. GAPDH was used as the inner control. (B) sM8α-His fusion protein was significantly overexpressed in LNCaP-sM8α cells. (C) The expression of p-JNK protein was significantly reduced in LNCaP-sM8α cells compared with LNCaP and LNCaP-NC cells. However, there were no significant changes in the expression of (D) p-ERK1/2 and (F) p-p38 or (E) full-length TRPM8 protein. (G) MMP-2 was activated, as indicated by upregulation of 64-kDa MMP-2 and downregulation of 72-kDa MMP-2 in LNCaP-sM8α cells. (H) There were no remarkable differences in the expression of MMP-9 in LNCaP, LNCaP-NC and LNCaP-sM8α cells. *P<0.05. NC, negative control; sM8α, short transient receptor potential melastatin 8α; TRPM8, transient receptor potential melastatin 8; p-ERK, phosphorylated-extracellular signal-regulated kinase 1/2; p-JNK, phosphorylated-c-Jun N-terminal kinase; MMP, matrix metalloproteinase; GAPDH, glyceraldehyde phosphate dehydrogenase.

    Article Snippet: Primary antibodies against human TRPM8 (rabbit polyclonal; catalog no. ACC-049; Alomone Labs, Jerusalem, Israel; 1:500 dilution), MMP-2 (rabbit monoclonal IgG; catalog no. 13132; Cell Signaling Technology, Inc., Danvers, MA, USA; 1:1,000 dilution), MMP-9 (rabbit monoclonal IgG; catalog no. 13667, Cell Signaling Technology, Inc.; 1:1,000 dilution), GAPDH (rabbit polyclonal IgG; catalog no. sc-25778, Santa Cruz Biotechnology, Inc., Dallas, TX, USA; 1:1000 dilution), MAPK family [rabbit anti-p38, ERK1/2 (p44/42) and JNK (catalog no. 9926), and phospho (p-)p38, p-ERK1/2 and p-JNK (catalog no. 9910); Cell Signaling Technology, Inc.] and His-tag (mouse monoclonal IgG2a; catalog no. D291-3, Medical & Biological Laboratories Co., Ltd., Nagoya, Japan) were applied overnight at 4°C.

    Techniques: Western Blot, Over Expression, Expressing, Negative Control